Reagent D eliminates DNA from dead cells to prevent false-positive PCR results in food safety testing. This light-sensitive reagent penetrates dead cell membranes and binds to DNA upon light exposure, blocking PCR amplification. Validated for testing Enterobacteriaceae, Salmonella, Legionella, and Vibrio in various food matrices including instant milk formula and dairy products. Compatible with Hygiena's DNA isolation kits, Reagent D ensures accurate detection of viable microorganisms in food safety and environmental monitoring applications.